Protein profiler module

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In 2001, the Human Genome Project completed the sequencing of all human genes. Far from closing the book, this body of work opened up a whole new field, proteomics, which asks a far more important question - what do our genes do? Genes encode proteins, and proteins determine an organism s form, function, and phenotype, and as such, are the raw materials of natural selection. Proteomics is the study of the function and structure of proteins and their interaction with each other and their environment. The Protein Profiler Kit moves beyond DNA and allows students to employ one of the most widely used techniques in biotechnology research protein gel electrophoresis.

The Protein Profiler Kit allows students to explore evolution at the molecular level using the central framework of biology. Students generate protein profiles from the muscles of distantly and closely related species of fish and compare and contrast these profiles to test the hypothesis that protein profiles are indicators of genetic and evolutionary relatedness: DNA > RNA > Protein > Trait

Changes in proteins reflect changes in the gene pool. Muscle protein mostly consists of actin and myosin, but numerous other proteins also make up muscle tissue. While actin and myosin are highly conserved across all animal species, other muscle proteins exhibit more variation even among closely related species. Variations between organisms protein profiles reflect physiological adaptations to different environments, but they originate as chance DNA mutations. Such random mutation events, if favourable, persist through the natural selection process and contribute to the evolution of species with new specialised functions: Mutation > Variation > Specialisation > Speciation > Evolution

The Protein Profiler Kit is open-ended and inquiry-based. Students are asked: Can molecular data reveal similarities and differences among species? In their analyses, students compare similarities and differences in their fish protein profiles, create evolutionary trees (cladograms) from their own gel electrophoresis data, and compare their results to published evolution data. Students are asked: Do the phylogenetic data agree? Does molecular evidence support or refute the theory of evolution? Why or why not? What explanations can you suggest?

Key Kit Features
● Explore evolution
● Study protein structure and function
● Apply protein electrophoresis
● Construct cladograms
● Complete in three 45-minute lab sessions

NB: You will also need 15% polyacrylamide gels or 4-20% Tox polyacrylamide gels or low melt agarose gels and electrophoresis chambers.

Spare 3:
EPE120580 | Protein Profiler Temp Sensitive Reagents
EPE120590 | Western Blot Temp Sensitive Reagents
DescriptionProtein Profiler Module
● 1 Prot/Elect pipet tips for gel loading, rack
● 60 Fliptop Micro test tubes, 1.5 ml
● 50 Screwcap micro test tubes, 1.5 ml
● 3 Disposable plastic transfer pipettes, packs
● 1 Laemmli sample buffer, 30 ml
● 1 Precision Plus Protein™ Kaleidoscope™ standards, 50 µl, prestained vial
● Electrophoresis buffer, 10x tris/glycine/SDS, 1L bottle
● 2 Bio-Safe Coomassie stain 100ml bottles
● 1 Actin and myosin standards, 500 µg, lyophilized vial
● 1 Dithiothreitol (DTT), 0.3g vial
● 1 Curriculum/Instruction manual