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Bio-Rad Comparative Proteomics Kits: Combined Kit

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Protein electrophoresis is principally performed using vertical electrophoresis apparatus and polyacrylamide gels; however, horizontal electrophoresis apparatus and agarose gels may be employed using a specifically designed protocol included in the kit curriculum. The protein profiler kit is a stand-alone activity, but was developed in conjunction with another kit (Comparative Proteomics Kit II: Western Blot Module) that takes the activity to the next level. The western blot kit enables students to use the proteomics techniques of Western blotting and immunodetection to identify a single muscle protein, myosin, out of the hundreds of muscle proteins in the muscle proteome. Each kit supports 32 students.
If using gel electrophoresis you will need ready gels or comparable 15% acrylamide gels, you will also need (for the protein profiler module) fish samples (5-8 types), horizontal gel electrophoresis chamber, suitable power supply, agarose, adjustable micropipettes (2-20 µl), pipette tips, acetic acid, ethanol, water bath.

Kit 1 - Protein Profiler Module
See EPE120080 for kit description
Kit 2 - Western Blot Module
See EPE120090 for kit description

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Associated Products

Bio-Rad Comparative Proteomics Kits: Protein Profiler Module

Catalogue Code: EPE120080
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Catalogue Price: £144.69

Bio-Rad Comparative Proteomics Kits: Western Blot Module

Catalogue Code: EPE120090
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DescriptionCombined Kit
Kit 1 - Protein Profiler Module
Prot/Elect pipet tips for gel loading, 1 rack
Fliptop Micro test tubes, 1.5 ml, 2 packs
Screwcap micro test tubes, 1.5 ml, 1 pack
Disposable plastic transfer pipets, 3 packs
Laemmli sample buffer, 30 ml , 1 bottle
Precision Plus Protein™ Kaleidoscope™ standards, 50 µl, prestained, 1 vial
Electrophoresis buffer, 10x Tris/glycine/SDS, 1 L, 1 bottle
Bio-Safe Coomassie stain, 200 ml, 2 bottles
Actin and myosin standards, 500 µg, lyophilized, 1 vial
Dithiothreitol (DTT), 256 mg, 1 vial
Gel staining trays, 4
Foam floats, 8
Curriculum/Instruction manual

Kit 2 - Western Blot Module
Primary antibody (anti-myosin light chain mouse monoclonal), lyophilized, 1 vial
Secondary antibody (goat anti-mouse polyclonal antibody
conjugated to horseradish peroxidase (HRP)), lyophilized, 1 vial
HRP color detection reagent A, 1 bottle
HRP color detection reagent B, 1 vial
Buffer, 10x Tris/glycine, 1 bottle
Nonfat dry milk blocker, 1 pack
10x Phosphate buffered saline (PBS), 2 bottles
10% Tween 20, 1 bottle
Nitrocellulose membranes, 0.45 µm, 8 sheets
Blotting paper, 16 sheets
Reagent tubes, 1 pack
Curriculum/Instruction manual