GM Food Investigator Kit

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Students engage in a complete investigation in which they gather sample food items from the grocery store, extract DNA from the samples, amplify the DNA using PCR, and use gel electrophoresis to identify the presence or absence of amplified GMO sequences. The GMO Investigator kit uses PCR and DNA electrophoresis to test for the presence of two different GMO-associated DNA sequences: the 35S promoter of the cauliflower mosaic virus, and the terminator of the nopaline synthase gene of Agrobacterium tumefaciens. These DNA sequences are present in most (>85%) of the GM crops that are approved for distribution worldwide. In addition, the integrity of the plant DNA extracted from food is tested by using PCR to amplify a section of the photosystem II chloroplast gene that is common to most higher plants. The kit allows a guided inquiry approach to this exercise. Students conduct sophisticated scientific procedures employing multiple levels of control that allow them to assess the validity of their results. They determine the presence or absence of GMO sequences in their test food and answer the questions: did we successfully extract DNA, did our PCR work as expected, and do we have contamination? Kit contains sufficient materials for 8 student workstations (2-4 students per workstation).
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DescriptionGMO Food Investigator Kit
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