Crime Scene Investigator PCR Basics™ kit

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Using real-world forensic lab techniques, students learn which human DNA sequences are used for crime scene, missing person, mass disaster, and paternity investigations. Using the polymerase chain reaction (PCR) and DNA electrophoresis, students determine the genotypes of five DNA samples and experience how real-world crime labs solve mysteries. DNA profiling determines the exact genotype of a DNA sample and distinguishes one human being from another by identifying a DNA 'bar code' that is unique to every individual.
There are over 3 billion bases in the human genetic blueprint, and more than 99.5% of them do not vary between human beings. Within the variant areas of the genome exist unique polymorphic sequences called short tandem repeats (STRs) of uncertain biological function. It is these DNA sequences that are used in forensic applications. The DNA samples contained in this introductory PCR kit are plasmids that have been engineered to mimic the natural variations in human DNA that exist between one human being and another at a single STR locus.
Students determine the genotypes of five samples and perform a simple statistical analysis on their own results to solve a forensic mystery.
Kit contains sufficient materials for 12 student workstations (2–4 students per workstation).
Please note that you will also require:
• Horizontal gel electrophoresis chambers
• Adjustable micropipettes (2–20 μl, 20–200μl)
• Pipette tips, aerosol barrier (2–20 μl, 20–200μl)
• Microcentrifuges
• Thermal cycler
• Power supplies Gel Staining Trays
DescriptionPCR Basics™ Kit