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Bio-Rad pGLO Bacterial Transformation Kit

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Catalogue Price: £101.54
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Genetic engineering is the process of manipulating the genetic material of an organism - often to include the DNA from a foreign organism. In this activity, students transform bacteria by introducing a gene from a bioluminescent jellyfish. They use the same procedure for creating 'designer proteins' that has led to the explosion of new health treatments, agricultural applications, and environmental solutions. Jellyfish genes make the invisible, visible! With this kit, students transform bacteria with a gene from the bioluminescent jellyfish Aequorea victoria. The pGLO plasmid is constructed with the jellyfish gene that encodes Green Fluorescent Protein (GFP), an antibiotic-resistance gene that encodes beta-lactamase protein and the AraC gene encoding a regulator protein that turns the GFP gene on and off. Bacteria transformed with pGLO are selected by ampicillin resistance, and, when induced to express GFP, the bugs glow fluorescent green under UV light! Gene regulation. With pGLO, students analyse the growth of bacteria on various media and examine the roles that external and internal factors play in gene regulation. Gene expression in all organisms is carefully regulated to allow adaptation to differing conditions and to prevent wasteful production of proteins. The bacterial genes encoding the enzymes needed to metabolise the simple sugar arabinose are a perfect example. A promoter region upstream of these genes acts as molecular on/off switch that regulates their expression. The genes are activated only when arabinose is present in the environment. The pGLO plasmid incorporates the arabinose promoter, but the genes involved in the breakdown of arabinose have been replaced with the jellyfish gene encoding GFP. When bacteria transformed with pGLO are grown in the presence of arabinose, the GFP gene switches on, causing the bacteria to express GFP and to fluoresce brilliant green. When students genetically reengineer bacteria with the genes from a bioluminescent jellyfish they never forget the central mantra of molecular biology: DNA > RNA > Protein > Trait 崱 Green Fluorescence! This kit supports 32 students.
Key Kit Features:
Transform bacteria with jellyfish gene
• Turn the modified genes on or off
• Study gene regulation
Complete in two 45 minute sessions.
DescriptionpGLOª_ Bacterial Transformation Kit
UN Class//