Analysis of lambda DNA kit

Catalogue Code:

EPE120140
Pack Quantity:
EACH
Availability:
Catalogue Price:
£142.18

Description

In this kit, students use three different restriction enzymes to digest genomic DNA samples of the lambda bacteriophage. The lambda genome has approximately 48,000 base pairs. Each restriction enzyme will cut the lambda DNA several times generating fragments of different sizes which are separated by agarose gel electrophoresis and visualised using Bio-Rad's non-toxic Fast Blast DNA Stain. Lambda DNA comes from a bacterial virus, or bacteriophage, which attacks bacteria by injecting them with its nucleic acid. Once inside, Lambda DNA hijacks the bacterial cellular machinery and replicates itself until the cells burst, releasing millions more bacteriophage to carry out the same infection process. Bacteriophage lambda is harmless to humans and other eukaryotic organisms, and therefore makes an excellent source of DNA for experimental study.
Kit contains sufficient materials for 8 student workstations (2–4 students per workstation).
Please note that you will also require:
• Horizontal gel electrophoresis chambers
• Adjustable micropipettes (2–20 μl, 20–200μl)
• Pipette tips (2–200μl)
• Power supplies
DescriptionAnalysis of Lambda DNA Kit
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